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SRX1123792: GSM1835812: RNA-Seq_E8.5 G9aKO-Rep2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 56.4M spots, 2.8G bases, 1.6Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Study of the impact of G9a on DNA methylation in mouse embryonic cells [seq]
PRJNA291405 • SRP061767 • All experiments • All runs
show Abstracthide Abstract
We explored the role of the lysine-methyltrasferase G9a in the control of DNA methylation during mouse embryogenesis. We provide maps of cytosine methylation by MeDIP and RRBS in G9a-deficient mouse embryos and ES cells, as well as ChIP-Seq profiles for H3K9me2 in embryos and RNA-Seq expression profiles in G9a-deficient embryos. Overall design: We prepared RRBS libraries from single embryos and mapped DNA methylation by RRBS in four WT and three G9aKO E8.5 embryos. We prepared RNA-Seq libraries from RNAs of single embryos and mapped the transcriptome of two WT and two G9aKO E8.5 embryos. We prepared H3K9me2 ChIP-Seq libraries from three independant ChIP experiments on eight pooled E8.5 embryos.
Sample: RNA-Seq_E8.5 G9aKO-Rep2
SAMN03943755 • SRS1016215 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted from the embryos with the RNeasy Protect Mini Kit (Qiagen). RNA-Seq libraries were prepared with the Ovation RNA-Seq System V2 (NuGEN) according to the manufacturer’s instructions.
Experiment attributes:
GEO Accession: GSM1835812
Links:
Runs: 1 run, 56.4M spots, 2.8G bases, 1.6Gb
Run# of Spots# of BasesSizePublished
SRR213343556,441,9432.8G1.6Gb2015-11-13

ID:
1639597

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